Surgery for Obesity and Related Diseases
Volume 6, Issue 5 , Pages 532-537 , September 2010

Pancreatic islet isolation after gastric bypass in a rat model: technique and initial results for a promising research tool

  • Patrick Gatmaitan, M.D.

      Affiliations

    • Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio
    • ,
  • Hazel Huang, M.S.

      Affiliations

    • Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio
    • ,
  • Joseph Talarico, M.D.

      Affiliations

    • Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio
    • ,
  • Fady Moustarah, M.D., M.P.H.

      Affiliations

    • Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio
    • ,
  • Sangeeta Kashyap, M.D.

      Affiliations

    • Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio
    • ,
  • John P. Kirwan, Ph.D.

      Affiliations

    • Department of Pathobiology, Cleveland Clinic, Cleveland, Ohio
    • ,
  • Philip R. Schauer, M.D., F.A.S.M.B.S.

      Affiliations

    • Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio
    • ,
  • Stacy A. Brethauer, M.D.

      Affiliations

    • Bariatric and Metabolic Institute, Cleveland Clinic, Cleveland, Ohio
    • Corresponding Author InformationCorrespondence: Stacy A. Brethauer, M.D. Bariatric and Metabolic Institute, Cleveland Clinic, 9500 Euclid Avenue, M61, Cleveland, OH 44195

Received 8 May 2009 ,Revised 9 March 2010 ,Accepted 18 May 2010.

References 

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  9. Rubino F, Forgione A, Cummings DE, et al. The mechanism of diabetes control after gastrointestinal bypass surgery reveals a role of the proximal small intestine in the pathophysiology of type 2 diabetes. Ann Surg. 2006;244:741–749
  10. Rubino F, Marescaux J. Effect of duodenal-jejunal exclusion in a non-obese animal model of type 2 diabetes: a new perspective for an old disease. Ann Surg. 2004;239:1–11
  11. Korner J, Bessler M, Inabnet W, et al. Exaggerated glucagon-like peptide-1 and blunted glucose-dependent insulinotropic peptide secretion are associated with Roux-en-Y gastric bypass but not adjustable gastric banding. Surg Obes Relat Dis. 2007;3:597–601
  12. le Roux CW, Aylwin SJ, Batterham RL, et al. Gut hormone profiles following bariatric surgery favor an anorectic state, facilitate weight loss, and improve metabolic parameters. Ann Surg. 2006;243:108–114
  13. le Roux CW, Welbourn R, Werling M, et al. Gut hormones as mediators of appetite and weight loss after Roux-en-Y gastric bypass. Ann Surg. 2007;246:780–785
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  15. Baggio LL, Drucker DJ. Biology of incretins: GLP-1 and GIP. Gastroenterology. 2007;132:2131–2157
  16. Meirelles K, Ahmed T, Culnan DM, et al. Mechanisms of glucose homeostasis after Roux-en-Y gastric bypass surgery in the obese, insulin-resistant Zucker rat. Ann Surg. 2009;249:277–285
  17. Kashyap SR, Daud S, Kelly KR, et al. Acute effects of gastric bypass versus gastric restrictive surgery on beta-cell function and insulinotropic hormones in severely obese patients with type 2 diabetes. Int J Obes (Lond) Epub. 2009 Dec 22;
  18. Clements RH, Gonzalez QH, Long CI, et al. Hormonal changes after Roux-en-Y gastric bypass for morbid obesity and the control of type-II diabetes mellitus. Am Surg. 2004;70:1–5
  19. Flatt PR. Effective surgical treatment of obesity may be mediated by ablation of the lipogenic gut hormone gastric inhibitory polypeptide (GIP): evidence and clinical opportunity for development of new obesity-diabetes drugs?. Diab Vasc Dis Res. 2007;4:151–153
  20. Laferrere B, Heshka S, Wang K, et al. Incretin levels and effect are markedly enhanced 1 month after Roux-en-Y gastric bypass surgery in obese patients with type 2 diabetes. Diabetes Care. 2007;30:1709–1716
  21. Meguid MM, Ramos EJ, Suzuki S, et al. A surgical rat model of human Roux-en-Y gastric bypass. J Gastrointest Surg. 2004;8:621–630
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  23. Cummings BP, Digitale EK, Stanhope KL, et al. Development and characterization of a novel rat model of type 2 diabetes mellitus: the UC Davis type 2 diabetes mellitus UCD-T2DM rat. Am J Physiol Regul Integr Comp Physiol. 2008;295:R1782–R1793

 Supported in part by a research grant from Ethicon-Endosurgery.

 This study was awarded the Karl Storz Endoscopy-America Bariatric Surgery Fellow Scholarship Award at the American Society for Metabolic and Bariatric Surgery 26th Annual Meeting

PII: S1550-7289(10)00526-5

doi: 10.1016/j.soard.2010.05.018

Surgery for Obesity and Related Diseases
Volume 6, Issue 5 , Pages 532-537 , September 2010

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