Abstract
Background
The changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when
weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown.
Objectives
To investigate the changes produced in SAT gene expression of morbidly obese women
when their weight loss stabilizes 2 years after RYGB.
Setting
University hospital.
Methods
SAT biopsies of the periumbilical area were taken before and 2 years after RYGB. Gene
expression levels were assessed by microarray analysis and significant differences
in gene expression were validated by real-time quantitative polymerase chain reaction.
The findings were also confirmed in an independent population of morbidly obese women.
Results
Microarray analysis revealed that the overexpressed differentially expressed genes
have a prominent role in the pathways involved in biosynthetic processes, especially
lipid or carboxylic ones (stearoyl-Coenzyme A desaturase-1, fatty acid desaturase-1,
fatty acid elongase-6, ATP citrate lyase, fatty acid synthase, lipin-1, monoacylglycerol
O-acyltransferase, patatin-like phospholipase domain containing-3, phosphate cytidylyltransferase-2,
cholesteryl ester transfer protein, transmembrane 7 superfamily member 2, pyruvate
carboxylase, and glycogen synthase 2). Most of the underexpressed differentially expressed
genes are related with immune system and inflammation processes (immune responses,
response to stress, cell death, regulation of biological quality, immune effector
process, the response to endogenous stimulus, and the response to other types of stimulus).
Conclusion
An improvement of the SAT inflammatory and immune profile and an induction of genes
involved in the regulation of lipid metabolism are shown when weight loss stabilizes
2 years after RYGB. Most of the genes shown are clearly linked to obesity and other
metabolic disorders.
Keywords
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Article info
Publication history
Published online: July 13, 2015
Identification
Copyright
© 2016 American Society for Bariatric Surgery. Published by Elsevier Inc. All rights reserved.
