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Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue

  • Author Footnotes
    1 JJG-P and CG-R contributed equally to this work.
    Juan José González-Plaza
    Footnotes
    1 JJG-P and CG-R contributed equally to this work.
    Affiliations
    Department of Genomic and Personalized Medicine, Center for Life Sciences, Nazarbayev University, Astana, Kazakhstan
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  • Author Footnotes
    1 JJG-P and CG-R contributed equally to this work.
    Carolina Gutiérrez-Repiso
    Footnotes
    1 JJG-P and CG-R contributed equally to this work.
    Affiliations
    Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain
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  • Sara García-Serrano
    Correspondence
    Correspondence: Eduardo García-Fuentes or Sara García-Serrano, Laboratorio de Investigación, Hospital Civil, Plaza del Hospital Civil s/n, 29009 Málaga, Spain.
    Affiliations
    Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain

    CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Málaga, Spain
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  • Francisca Rodriguez-Pacheco
    Affiliations
    Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain

    CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Málaga, Spain
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  • Lourdes Garrido-Sánchez
    Affiliations
    Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain

    CIBER Fisiopatología de la Obesidad y Nutrición Asociadas (CIBEROBN), Málaga, Spain
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  • Concepción Santiago-Fernández
    Affiliations
    Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Virgen de la Victoria, Málaga, Spain

    CIBER Fisiopatología de la Obesidad y Nutrición Asociadas (CIBEROBN), Málaga, Spain
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  • Juan García-Arnés
    Affiliations
    Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain
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  • Francisco J. Moreno-Ruiz
    Affiliations
    Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain
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  • Alberto Rodríguez-Cañete
    Affiliations
    Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain
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  • Eduardo García-Fuentes
    Correspondence
    Correspondence: Eduardo García-Fuentes or Sara García-Serrano, Laboratorio de Investigación, Hospital Civil, Plaza del Hospital Civil s/n, 29009 Málaga, Spain.
    Affiliations
    Unidad de Gestión Clínica de Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario, Málaga, Spain

    CIBER Fisiopatología de la Obesidad y Nutrición Asociadas (CIBEROBN), Málaga, Spain
    Search for articles by this author
  • Author Footnotes
    1 JJG-P and CG-R contributed equally to this work.

      Abstract

      Background

      The changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown.

      Objectives

      To investigate the changes produced in SAT gene expression of morbidly obese women when their weight loss stabilizes 2 years after RYGB.

      Setting

      University hospital.

      Methods

      SAT biopsies of the periumbilical area were taken before and 2 years after RYGB. Gene expression levels were assessed by microarray analysis and significant differences in gene expression were validated by real-time quantitative polymerase chain reaction. The findings were also confirmed in an independent population of morbidly obese women.

      Results

      Microarray analysis revealed that the overexpressed differentially expressed genes have a prominent role in the pathways involved in biosynthetic processes, especially lipid or carboxylic ones (stearoyl-Coenzyme A desaturase-1, fatty acid desaturase-1, fatty acid elongase-6, ATP citrate lyase, fatty acid synthase, lipin-1, monoacylglycerol O-acyltransferase, patatin-like phospholipase domain containing-3, phosphate cytidylyltransferase-2, cholesteryl ester transfer protein, transmembrane 7 superfamily member 2, pyruvate carboxylase, and glycogen synthase 2). Most of the underexpressed differentially expressed genes are related with immune system and inflammation processes (immune responses, response to stress, cell death, regulation of biological quality, immune effector process, the response to endogenous stimulus, and the response to other types of stimulus).

      Conclusion

      An improvement of the SAT inflammatory and immune profile and an induction of genes involved in the regulation of lipid metabolism are shown when weight loss stabilizes 2 years after RYGB. Most of the genes shown are clearly linked to obesity and other metabolic disorders.

      Keywords

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