Highlights
- 1.cg06093355, cg00294552, cg00753924, and cg17092065 were identified.
- 2.The nomogram accurately predicted one-year EWL% after LSG.
- 3.The model provided new insight into the postoperative assessment of bariatric surgery.
Abstract
Background
Laparoscopic sleeve gastrectomy (LSG) is a crucial surgical procedure for patients
with obesity. However, epigenetic research in LSG is still in its infancy from the
perspective of adipogenesis.
Objectives
This work aims to develop a model to predict one-year excess weight loss% (EWL)% following
LSG in Chinese patients with obesity by examining the DNA methylation profiles of
intraoperative visceral fat.
Setting
University hospital, Beijing, China.
Methods
Firstly, we classified patients with obesity as either the satisfied group or unsatisfied
group depending on whether their EWL% was 50% or higher at one year following LSG.
After that, we analyzed differentially methylated sites (DMSs) between the satisfied
group and unsatisfied group. DMSs were mapped to the corresponding differentially
methylated genes (DMGs). Then, we took the intersection of adipogenesis-related genes
and DMGs, and obtained adipogenesis-related DMSs. Next, hub methylation sites were
identified by LASSO analysis. Finally, a nomogram was developed to predict EWL% of
Chinese patients with obesity at one-year following LSG.
Results
A total of 26 patients with obesity were enrolled in the study, including 13 in the
satisfied group and 13 in the unsatisfied group. A total of 16 genes and 31 DMSs were
involved in the adipogenesis signaling pathway. Finally, four hub methylation sites
(cg06093355, cg00294552, cg00753924, and cg17092065) were identified and a predictive
nomogram was established.
Conclusions
The predictive nomogram based on methylation sites including cg06093355, cg00294552,
cg00753924, and cg17092065 can predict EWL% at one-year following LSG in Chinese patients
with obesity efficiently.
Keywords
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References
- Worldwide trends in body-mass index, underweight, overweight, and obesity from 1975 to 2016: a pooled analysis of 2416 population-based measurement studies in 128.9 million children, adolescents, and adults.Lancet. 2017; 390: 2627-2642
- Obesity: Epidemiology, Pathophysiology, and Therapeutics.Frontiers in Endocrinology. 2021; 12706978
- Obesity Phenotypes, Diabetes, and Cardiovascular Diseases.Circulation Research. 2020; 126: 1477-1500
- Pro-inflammatory cytokines: The link between obesity and osteoarthritis.Cytokine & Growth Factor Reviews. 2018; 44: 38-50
- Benefits and Risks of Bariatric Surgery in Adults.JAMA. 2020; 324: 879
- Treatment of Obesity.Circulation Research. 2016; 118: 1844-1855
- What variables are associated with successful weight loss outcomes for bariatric surgery after 1 year?.Surgery for Obesity and Related Diseases. 2014; 10: 697-704
- Long-Term Weight Loss Results, Remission of Comorbidities and Nutritional Deficiencies of Sleeve Gastrectomy (SG), Roux-En-Y Gastric Bypass (RYGB) and One-Anastomosis Gastric Bypass (OAGB) on Type 2 Diabetic (T2D) Patients.Int J Environ Res Public Health. 2020 Oct 20; 17: 7644
- Genetic Determinants of Weight Loss After Bariatric Surgery.Obes Surg. 2019 Aug; 29: 2554-2561
- Obesity-Related Epigenetic Changes After Bariatric Surgery.Frontiers in Endocrinology. 2019; 10: 232
- The Effect of Metabolic and Bariatric Surgery on DNA Methylation Patterns.Current Atherosclerosis Reports. 2017; 19: 40
- Adipogenesis and metabolic health.Nature Reviews Molecular Cell Biology. 2019; 20: 242-258
- Harnessing adipogenesis to prevent obesity.Adipocyte. 2019; 8: 98-104
- Contribution of adipogenesis to healthy adipose tissue expansion in obesity.Journal of Clinical Investigation. 2019; 129: 4022-4031
- Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers.Signal Transduction and Targeted Therapy. 2019; 4: 55
- Analysing and interpreting DNA methylation data.Nature Reviews Genetics. 2012; 13: 705-719
- DNA Methylation in Cancer and Aging.Cancer Research. 2016; 76: 3446-3450
- The diverse roles of DNA methylation in mammalian development and disease.Nature Reviews Molecular Cell Biology. 2019; 20: 590-607
- The Role of Epigenetics in Autoimmune/Inflammatory Disease.Frontiers in Immunology. 2019; 10: 1525
- DNA methylation markers in obesity, metabolic syndrome, and weight loss.Epigenetics. 2019; 14: 421-444
- An overview about DNA methylation in childhood obesity: Characteristics of the studies and main findings.Journal of Cellular Biochemistry. 2020; 121: 3042-3057
- BMI, leisure-time physical activity, and physical fitness in adults in China: results from a series of national surveys, 2000–14.The Lancet Diabetes & Endocrinology. 2016; 4: 487-497
- Molecular Regulation of Adipogenesis.Annual Review of Cell and Developmental Biology. 2000; 16: 145-171
- Sleeve gastrectomy and type 2 diabetes mellitus: a systematic review.Surgery for Obesity and Related Diseases. 2010; 6: 707-713
- Prevention of Weight Regain Following Bariatric Surgery.Current Obesity Reports. 2015; 4: 198-206
- Trends in Weight Regain Following Roux-en-Y Gastric Bypass (RYGB) Bariatric Surgery.Obesity Surgery. 2015; 25: 1474-1481
- Factors associated with weight regain post-bariatric surgery: a systematic review.Surgical Endoscopy. 2021; 35: 4069-4084
- Bariatric Surgery and Precision Nutrition.Nutrients. 2017; 9: 974
- Epigenetics of obesity.Current Opinion in Clinical Nutrition and Metabolic Care. 2015; 18: 361-366
- EPAS1 Promotes Adipose Differentiation in 3T3-L1 Cells.Journal of Biological Chemistry. 2004; 279: 40946-40953
- Protection from Obesity and Diabetes by Blockade of TGF-β/Smad3 Signaling.Cell Metabolism. 2011; 14: 67-79
- SMAD2 and SMAD3 differentially regulate adiposity and the growth of subcutaneous white adipose tissue.The FASEB Journal. 2021; 35e22018
- TET1 promotes RXRα expression and adipogenesis through DNA demethylation.Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2021; 1866158919
- Epigenetic Gene Promoter Methylation at Birth Is Associated With Child’s Later Adiposity.Diabetes. 2011; 60: 1528-1534
- Associations of markers in 11 obesity candidate genes with maximal weight loss and weight regain in the SOS bariatric surgery cases.International Journal of Obesity. 2011; 35: 676-683
- Development and Validation of a Simple-to-Use Nomogram for Predicting 5-, 10-, and 15-Year Survival in Asymptomatic Adults Undergoing Coronary Artery Calcium Scoring.JACC: Cardiovascular Imaging. 2018; 11: 450-458
- Pre-surgical stress and social support predict post-surgical percent excess weight loss in a population of bariatric surgery patients.Psychology, Health & Medicine. 2020; 25: 1258-1265
D EUSEBIO C, BOSCHETTI S, RAHIMI F, et al. What predicts the unsuccess of bariatric surgery? An observational retrospective study. Journal of Endocrinological Investigation, 2021,44(5): 1021-1029.
- Weight loss after one-anastomosis/mini-gastric bypass – The impact of biliopancreatic limb: A retrospective cohort study.Journal of Research in Medical Sciences. 2020; 25: 5
- Early weight loss after laparoscopic sleeve gastrectomy predicts midterm weight loss in morbidly obese Asians.Surgery for Obesity and Related Diseases. 2017; 13: 1966-1972
- How To Build and Interpret a Nomogram for Cancer Prognosis.Journal of Clinical Oncology. 2008; 26: 1364-1370
Article info
Publication history
Accepted:
February 24,
2023
Received in revised form:
January 12,
2023
Received:
November 2,
2022
Publication stage
In Press Journal Pre-ProofFootnotes
Abbreviations: 5' UTR: 5' untranslated region; ARG: Adipogenesis-related gene; AUC: area under the curve; BMI: body mass index; DCA: decision curve analysis; DMGs: differentially methylated genes; DMS: differentially methylated site; EPAS1: Endothelial PAS Domain Protein 1; EWL: excess weight loss; FL: fatty liver; IRB: Institutional Review Board; LASSO: least absolute shrinkage and selection operator; LSG: Laparoscopic sleeve gastrectomy; NR3C1: Nuclear Receptor Subfamily 3 Group C Member 1; ROC: receiver operating characteristic; RXRA: Retinoid X Receptor Alpha; SMAD3: SMAD Family Member 3; T2DM: type 2 diabetes mellitus; TSS1500: 200–1500 bases upstream of the transcription start site; TSS200: 0–200 methylation profile analysis in obesity 1441 bases upstream of the transcription start site; WHR: waist-to-hip ratio.
Data Availability Statement
All relevant data and information can be obtained from the corresponding author upon reasonable request.
Author Contributions
D.L. and N.Z. contributed to study conception and design. Z.L., G.C., Q.S., L.W., Q.W., and Z.W. contributed to data collection. Z.L., G.C., B.A., and Z.W. contributed to data analysis, and manuscript drafting. All authors were involved in the revision of the manuscript and approved the final version of the article.
Ethics approval and consent to participate
All procedures performed in studies involving human participants were following the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Conflicts of Interest
The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Funding
We received no external funding for this study.
Identification
Copyright
© 2023 American Society for Metabolic and Bariatric Surgery. Published by Elsevier Inc. All rights reserved.
